FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404565

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404565
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9204017
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGTT16450.042.303066145
CGCCGTT19300.033.427143145
CCGTATA19800.028.555906145
GACGATA36750.024.655806145
GTCCTAG35500.021.4484061
GCCGACC42000.021.056059145
CGACGAA33300.020.67978145
GTCTTAG34900.019.7393231
GTACTAG17800.019.5548861
CGTGTAT27050.019.294422145
TTATACC101850.019.073935145
GTGCTAG34700.019.0171761
GTCCTAA31450.018.2154771
GCTCTAG41850.018.1939891
GTTCTAG40250.018.0164091
TAGATCG14250.017.804087145
GTACTAA28400.017.6183341
GTATTAA46700.017.546711
GTATATA51550.017.3025671
GTATAGG25400.017.1297741
Produced by FastQC (version 0.11.5)