FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404566

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404566
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8766727
Sequences flagged as poor quality0
Sequence length35-76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG11300.023.8151761
GTATTAG19300.021.9879841
GTATATA42150.015.5522641
GTATTAA30450.015.4095251
GTACTAA18700.015.1289871
GTATAGG14600.015.1239321
GTCCTAG20300.014.6163861
GTGCTAG19500.014.154451
GCTCTAG22650.014.0138431
GTACTAT19150.013.8726541
GGTCTAA17100.013.7198841
GTATTAT36050.013.6857431
GTACAGG34600.013.6609871
GTTCTAG27350.013.6239811
TAGACTG24400.013.5748145
CCTATAC15000.013.5707343
GTCCTAA20050.013.4220181
GTATAAG21350.013.2511511
GCTCTAA23750.013.2195111
GTCTTAG21250.013.151151
Produced by FastQC (version 0.11.5)