FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404567

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404567
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8766727
Sequences flagged as poor quality0
Sequence length35-76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCTAG19500.017.20321
GTCCTAG18500.016.6375891
GTACTAG10550.016.3904041
GTACTAA20050.016.3863161
GTTCTAG25250.015.8879811
GTATATA39900.015.6883691
GTCTTAG20750.015.5001871
GTATTAA33400.015.3245361
GTCCTAA19650.015.1358911
GTGCTAA22950.014.767791
GTATAAG20050.013.7990041
GTATAGC13400.013.6786481
GTACATG30900.013.5425051
CTATACT23000.013.5032814
GTACATA29500.013.4818011
GTATTAG20800.013.46771051
GCTCTAG22200.013.0857441
GTGTTAA31500.013.0649721
GTACAGG34550.013.01271
GTCTACG7450.012.997921
Produced by FastQC (version 0.11.5)