FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404568

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404568
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8499204
Sequences flagged as poor quality0
Sequence length35-76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG20150.018.838331
GTATAGG15050.017.4261651
GTATTAA32000.015.5287761
GTTCTAA28150.015.32344151
GGTCTAA14200.015.3100611
TATACCG5350.014.8360825
GTCTTAG19550.014.8271361
GTACTAA18500.014.7360171
GTATTAG19500.014.3342551
GTACTAG12550.014.2982981
GTTCTAG25900.014.2563631
GTATATA37450.013.8217851
GTCTTAA26250.013.5404371
GTGCTAG20150.013.3580881
TCTATAC19900.013.3528863
GATCTAG16400.013.2562721
GTACAGG38050.013.2410651
GTATAAG19100.013.1890721
ATAGTAC15600.013.0516353
GTACTAC14300.013.0311411
Produced by FastQC (version 0.11.5)