FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404569

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404569
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8499204
Sequences flagged as poor quality0
Sequence length35-76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG97490.11470485941977625No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG11300.018.06021
GTATTAA32450.016.6287211
GTATTAG19150.015.5338321
GTACTAA17850.015.5024641
GTCTTAG19350.015.3732771
GTCCTAA19850.014.9860411
GTTCTAG25550.014.8919211
GTTCTAA26500.014.4885881
GTCCTAG20950.014.1991841
GTGCTAG20100.014.1112921
GTCCTAT19750.013.835951
GTATAGG13600.013.7342141
GCGCTAG3303.997593E-813.6263131
CCTATAC14250.013.5737623
GTATATA40250.013.3203231
GTATTAT34750.013.2387131
GTATACG5001.8189894E-1213.1441521
GGTCTAA17250.013.0338641
GTATAAG20800.012.9712021
TATACTG29000.012.9708825
Produced by FastQC (version 0.11.5)