FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404570

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404570
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8669213
Sequences flagged as poor quality0
Sequence length35-76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG19650.020.0177671
GTCCTAG21400.016.7684481
GTATATA39000.016.102011
GTACTAA18450.015.5222641
GTCTTAA28950.015.4941621
GTATAAG19500.015.2172841
GTGCTAG19250.014.6979391
GTATTAA32950.014.3462521
CTATACT22250.014.11224
GTACTAG10100.014.00670051
GTACATA28450.013.8259791
GTTCTAG24500.013.3792221
GTATATG30300.013.3234471
TCTATAC21000.013.3090353
GTCCTAA17550.012.9759791
GTATAGG16300.012.9126561
CTAGACT19350.012.8391084
GTCTTAG18850.012.8132711
GTCTAGA19000.012.7121141
TGCACTG54600.012.7026485
Produced by FastQC (version 0.11.5)