FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404571

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404571
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8669213
Sequences flagged as poor quality0
Sequence length35-76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG19750.018.3599971
GTCCTAA19750.016.7862821
GTCTTAG19250.016.6840921
GTACTAG10200.016.5899961
GTTCTAG27700.016.581441
GTGCTAG21600.015.828211
GTATTAA32450.015.7506111
GCGCTAG2959.336873E-915.2184951
GTCTTAA25500.014.7617111
GTATTAG19550.014.66161251
GGTCTAG15800.014.4257121
GGTCTAA19650.014.4112511
GCTCTAG22450.014.305961
GTATACG4403.6379788E-1214.127661
GCCCTAG18100.013.9281891
TCTAGAC18750.013.8021583
GTATATA38700.013.6530871
GTTCTAA31850.013.4450561
GTATATG31800.013.3575981
CCTATAC15250.013.34962753
Produced by FastQC (version 0.11.5)