FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404572

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404572
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8714137
Sequences flagged as poor quality0
Sequence length35-76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAA33900.017.3039041
GTATTAG21400.017.2530081
GTACTAA18250.016.4494441
GTACTAG10900.015.8284481
GTGCTAG16600.014.9664651
GTTCTAG21800.014.7204571
GCTCTAG23350.014.3344051
GTATATA39500.014.3265491
GGTCTAA16700.014.0503531
GTCTTAG18500.013.6158881
TGCACCG14700.013.6150885
CCCTTAG21300.013.6080071
GTCCTAG19250.013.4439031
GTCTAGG21250.013.3152631
GTACAGG35550.013.2976771
CAGTACT32300.013.1403654
GTCCTAA20250.012.9504061
GTCCTAT19300.012.8727111
GTATAGA26050.012.848691
GTATAGG14900.012.737121
Produced by FastQC (version 0.11.5)