FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404575

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404575
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6891613
Sequences flagged as poor quality0
Sequence length35-76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG16400.019.0496061
GTCCTAG17200.017.356311
GTATAGG11300.016.8955211
GTCCTAT16550.015.9405361
GTACTAG9400.015.8791751
GCTCTAG18400.015.8470641
GTTCTAG20400.015.48454951
GTATACG2958.722054E-915.2970861
GTACTAA17250.015.2936741
GTATTAA28450.015.0075651
GTATATA28700.014.9977861
GTCCTAA14650.014.6906641
GTATATG26100.014.6298391
GTCTTAA22350.014.4441771
CCTATAC13000.014.1330443
TATACTG23950.014.0268265
GTATAAA32550.013.9703621
GTTCTAA24600.013.9697121
GGTCGCG2557.0462065E-613.6127911
GTCTAGT11050.013.5080771
Produced by FastQC (version 0.11.5)