FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404576

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404576
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6894303
Sequences flagged as poor quality0
Sequence length35-76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG9900.017.4866431
GTATTAG15350.016.2403641
GTATTAA28750.016.1375871
GTCTTAG16450.015.7858141
GTTCTAG20850.015.7757211
GTACTAA15550.015.5861651
GTCTTAA21600.015.3882461
GTATAGG13600.015.0205131
GTCCTAG16250.014.7016941
GCTAGAC9850.014.4118353
GTATAAG15500.014.2961771
GTTCTAA24100.014.2229531
GTATAGA19700.014.0603271
GTCTAGA15850.013.9804881
TCTAGAC16600.013.9745673
GTCTTAT19650.013.9199011
GTATTAT25650.013.7684311
GTCCTAA14600.013.7545621
GTACTAT14100.013.7511981
CTCCTAG24000.013.5608921
Produced by FastQC (version 0.11.5)