FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404577

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404577
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6894303
Sequences flagged as poor quality0
Sequence length35-76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG9200.017.7284561
GTCCTAG15100.017.236351
GTGCTAG13900.017.2264271
GTTCTAG22700.017.1219441
GTACTAA15000.016.6572061
GTCTTAG13350.016.3764691
GGTCTAG13000.016.2834871
GTATTAA27550.016.249091
GTCCTAA15700.015.6934931
GTACCGT3552.7284841E-1115.6059676
GTATATA28600.015.5311951
GTATTAG18150.014.9134771
GCTCTAG18100.014.7629491
TCTATAC16950.014.1102733
GTATTAT26100.013.6948611
CCTATAC12950.013.6507493
GTCTTAA23450.013.6146321
CCCCTAG15850.013.5744851
GTATAGG12800.013.5556691
GTATAAA35250.013.3887711
Produced by FastQC (version 0.11.5)