FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404578

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404578
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7124047
Sequences flagged as poor quality0
Sequence length35-76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG10450.018.5527251
GTATTAA27550.016.7134461
GTATACG3800.016.3992841
GTATTAG16950.015.7274021
GTACTAA18150.015.6413141
GTGCTAG16450.015.3635981
GTCTTAG16550.015.2707671
GTCCTAG18300.014.5671841
GTACAGG30550.014.5055681
GTTCTAA24350.014.502311
GTATAGG12550.014.3448361
TCTATAC18200.014.2676783
GTTCTAG20750.014.1819781
TCTAGAC17000.014.0528223
GTATATA30400.013.5521861
CTAGCGT2304.1360534E-513.548094
GTGCTAA20300.013.47308351
CTATACT20950.013.3864184
GTCTATA15800.013.3662241
GTCTTAT21300.013.3281611
Produced by FastQC (version 0.11.5)