FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404579

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404579
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7124047
Sequences flagged as poor quality0
Sequence length35-76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCTAG2350.022.1131041
GTACTAG10800.017.9634841
GTCCTAG17250.016.8700541
GTTCTAG21650.016.1618021
GTTCTAA24050.015.9894751
GTATATA30200.015.9453541
GTATTAA27500.015.8731871
GTCTTAG15700.015.666971
GTATTAG15100.014.9129211
GTATAGA19900.014.623541
GTCTTAA22900.014.3719081
GTGCTAA20400.014.26512051
CTAGACC13850.013.9965164
GTACTAT16400.013.7307991
TCTATAC17000.013.439493
GTACTAA17050.013.4105271
CCTATAC14200.013.4079423
GTGCTAG15800.013.3751611
GTCCTAA16850.013.3641011
CTATACT18900.013.1871934
Produced by FastQC (version 0.11.5)