FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404580

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404580
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7055282
Sequences flagged as poor quality0
Sequence length35-76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG9200.019.9462051
GTCCTAG17800.019.6459771
GTATAGG11500.016.860191
GCGCTAG1452.6515662E-416.7148441
GTATTAG15550.016.2541681
GTATAAG15250.016.119841
GTTCTAG20500.015.70729351
GTCTAGA16100.015.053741
GTACTAA15900.015.0253361
GTATTAT26150.014.6968261
GTATTAA28000.014.5913741
GGTCTAG13350.014.0050531
GTCTATA14350.013.9942091
GTCTTAA22000.013.8494421
GTGCTAG17050.013.8088261
GTCTTAG17100.013.768451
TAGTGCG3303.564128E-813.74599257
GTACATA22800.013.3634961
GGTCTAA13550.013.2872881
GTATATA32050.013.1796541
Produced by FastQC (version 0.11.5)