FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404583

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404583
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12772552
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG13050.017.6918011
GTCCTAG29800.015.9577091
GTATATA38100.014.7424741
GTATTAG23650.014.7162771
GTTCTAG35250.014.4680611
GTACTAA21850.013.8783861
GTATAGG20050.013.7493881
GTGCTAG30600.013.7387161
GGTCTAG24000.013.3530191
CTATACT26950.013.2694454
TACGCGA2902.0122498E-613.0569232
GTCTAGA28600.012.7716671
GTCTTAG30000.012.7499791
GTATTAA38300.012.6860991
TCTAGAC30250.012.3966843
GTCTTAA33100.012.3887271
GTCCTAT28550.012.3112391
GTACAGG55800.012.2892851
CTAGACT28250.012.1719394
GTGCTAA28600.012.16922951
Produced by FastQC (version 0.11.5)