FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404584

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404584
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12403846
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG13800.017.689621
GTATTAG22500.017.2677271
GTCCTAG30600.015.2811751
GTCTTAG25750.014.153631
GTATATA38100.013.1754991
GTACATA31250.013.0929091
GTACTAT21150.012.8426891
CCTATAC18900.012.7345173
GTCTAGG28700.012.3394151
TCTAGAC27800.012.1206963
GTTCTAG33250.011.9951481
GTATTAT33000.011.8776391
GGTCTAA21750.011.8560831
TCTGTAC52500.011.7885253
CTAGACC24800.011.7846114
GTCCTAA25400.011.77673151
GCCCTAG28350.011.7640791
GTATAGG18450.011.7404151
GTCTATA21800.011.6711721
GTGCTAG29800.011.42242051
Produced by FastQC (version 0.11.5)