FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404586

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404586
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12554750
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG13800.016.6919671
GTATTAG24550.014.5644351
GTCCTAG28400.014.52695751
CTATACT27650.014.4238624
GTTCTAA36600.014.0903561
TAGTGCG6750.014.06837857
GTATATA39100.013.8049461
TCTATAC25400.013.6709743
GTGCTAG30850.013.150391
GCTCTAG36600.012.8691911
GTTCTAG33250.012.8215881
TACGCGA3002.9837047E-612.6061722
GTATTAA33350.012.5769621
GTACAGG54300.012.5365241
GTACAAG41150.012.5323711
GTGTAGG43450.012.5019891
CCTATAC19850.012.4704973
GTATAGG18150.012.3125651
GTACTAT22800.012.2141131
CCTGTAC56750.012.1770473
Produced by FastQC (version 0.11.5)