FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404588

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404588
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12698946
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG31400.018.5072361
GTCTTAG26600.016.0296691
GTACTAG14250.015.4436391
GTCCTAA23600.014.8618441
GTATTAG23200.014.3769991
GTTCTAG33550.014.2464541
GTATAGG20250.013.9242981
GTATATA40900.013.5358981
GTACTAA22800.013.4226921
TAGTGCG7400.013.30371757
GTATTAA33100.013.0896221
CTATACT26800.013.08801654
GTACAGG52300.013.0838591
TCTAGAC29750.012.9458613
GCTCTAG38000.012.8495891
GTATAAG22700.012.5729371
TCTAGGC38400.012.5370893
GTGCTAG29200.012.3649091
GTTCTAT33350.012.2697491
GCCCTAG30850.012.2608911
Produced by FastQC (version 0.11.5)