FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404589

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404589
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12698946
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG142700.11237153067664041No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG22000.016.5772511
GTCCTAG29350.016.5287651
GTGCTAG27950.014.8947391
GTACTAG13250.014.8009071
GTTCTAG33950.014.491911
GCTCTAG36450.013.49795151
GTATTAA34500.013.4630671
GGTCTAA24050.013.304461
GGTCTAG24450.013.2275181
CTATACT26700.012.8783884
GTACTAA22600.012.7879291
GTCTTAG26500.012.7235871
GTATTAT32800.012.587421
TCTATAC26500.012.4568053
GTTCTAT33550.012.3060341
GTATATA38850.012.22131
GTTCTAA34250.012.0545231
GTATAAG26750.011.9615791
GTCCTAA28650.011.8888521
GTCTAGA30150.011.8679421
Produced by FastQC (version 0.11.5)