FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404594

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404594
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6344630
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG19200.023.0370311
GTGCTAG22600.021.817171
GTACTAA20050.021.6987531
CGTCTTG41200.020.58851145
GGTCTAG21100.019.588011
GTACTAG12750.019.3361
GTATTAA32450.017.8761181
GTCCTAG23750.017.7077061
TACGCGA3352.8595072E-517.3152752
GTACTGG42200.017.0106411
GTATACG5552.1809683E-916.9843241
TGCCGTG53850.016.82909145
GACGCTA26650.016.594683145
GTTCTAG27200.016.2614351
GTATAGG16500.016.2598171
GTACAGG48300.016.2134171
CGTACCG4951.8680112E-716.1128255
GTGTAGG32450.016.0885071
TCTGTAC44750.016.0408133
GGGGGGG2920450.016.009542145
Produced by FastQC (version 0.11.5)