FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404597

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404597
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6323142
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGTT15850.031.559689145
GTATTAG22850.022.8608531
GTGCTAG23600.022.1343421
CGCCGTT16350.020.839771145
GACGATA31700.018.524164145
GCCGACC33400.018.015423145
GTACTAA20400.017.782221
GTACTAG11900.017.6806071
CGTGTAT22650.017.603836145
GTATTAA34650.017.5882321
CTATACT22500.017.4009194
CGACGAA25850.017.387775145
GTCTTAG24600.016.8107031
GGATTAG21600.016.794321
GTATTGG29850.016.7706891
GTCCTAA22200.016.6672271
GTATAGG16650.016.558291
GGTCTAG20600.016.5530031
GCTCTAG28150.016.4948251
GTTCTAG28150.016.2370951
Produced by FastQC (version 0.11.5)