FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404598

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404598
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6469780
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCTA48000.042.01497145
GTATTAG24400.021.5319141
GTCCTAG25650.020.0062641
GTATTAA43900.017.1164861
GTACTAA23400.016.4474561
GGGGGGG300450.016.031384145
GTTCTAG31700.015.6098881
TAGACCG4856.2700565E-815.1154215
TGTACCG8900.014.4148475
GCCTTAG23350.014.3896361
CTGTACT55250.014.3744694
GTATATA51850.014.3742461
GTATATG37250.014.2681281
GTTCTAA36550.014.2071051
CTATACT26450.014.0891534
GCTCTAG29150.013.8318221
CTTAACG8007.2759576E-1213.7455853
CTAGACC21150.013.5758874
GCCCTAG20700.013.5756771
GTCTTAG25250.013.5487941
Produced by FastQC (version 0.11.5)