FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404599

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404599
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6469780
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCTA46900.023.550821145
GTCCTAG27150.017.573361
GTATTAG25600.017.442671
GTACTAG14850.016.476451
GTTCTAG31550.016.0919571
GCTCTAG27650.015.7070021
TCTATAC28550.015.6362543
GTCCTAA26300.015.5829321
TAACTCG7600.00292766115.571429145
GTCTTAG26200.015.4089391
GTACTAT22500.015.2242391
GTGCTAG27050.015.1508731
ATAGTAC22750.014.7841813
CTAGACT23000.014.6234844
GTACTAA22750.014.5191931
GTTCTAA34500.014.3613751
TCTAGAC24800.014.3018513
GTATAGG17900.014.01073551
GTATATA51400.013.9236411
GTATTAA43800.013.82582951
Produced by FastQC (version 0.11.5)