FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404600

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404600
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6493518
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCTA48650.037.234432145
GTCCTAG27200.020.9187371
GTATTAG27100.020.3186421
GTCTTAG27800.018.9267081
GTATATA46850.017.6297281
GTTCTAG30350.017.336491
GGGGGGG281650.017.012573145
GTACTAA23550.016.6268711
GTATATG40100.016.1727161
GTATAGG19650.015.8792171
TCTAGAC25000.015.6627543
GTATTAA41900.015.6239631
GTCTTAT34250.015.5410421
ATAGTAC25050.015.1430063
TATACTG38350.014.359375
GTCTTAA33550.014.2240591
CTATACT27700.014.1368224
GTGCTAG24600.013.927541
GTATTAT43950.013.9207491
GTACAGA54550.013.79532051
Produced by FastQC (version 0.11.5)