FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404602

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404602
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6448419
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCTA50850.047.054485145
GTACTAG13800.019.945461
GTACTAA24650.017.8659521
GTATTAA43700.017.3560491
GTATATA48050.017.1850571
GTATTAG26000.015.9972811
GTTCTAG32050.015.8401991
GTGTACG8900.015.8069641
TATACTG37850.015.6756875
GTCTTAG25350.015.4423221
GGGGGGG253300.015.311973145
GTATAAG25150.014.8355091
GGTCTAG20050.014.64324951
GTCCTAG25650.014.5463181
GGTCTAA21900.014.5234511
GTCCTAA23900.014.0758751
CTAACCT26600.013.5672264
CTATACT28200.013.44817354
GCCCTAG21500.013.3711871
ATAGTAC24350.013.3134583
Produced by FastQC (version 0.11.5)