FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404604

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404604
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6371139
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCTA48750.049.503803145
GTACTAA22050.020.2066171
GTATTAA43400.018.1417581
CTATACT27250.017.6958184
GTATAGG20000.016.1742921
GTTCTAG31350.015.7698261
GGGGGGG297450.015.572362145
GTATATA47800.015.19492151
GTACTAG14200.015.0438531
CCTATAC18700.015.0139723
GTCCTAG24100.014.9421941
GTATTAG24900.014.7072441
GTCCTAT23100.014.5321571
GTCTTAA33350.014.4580811
GTGCTAG24800.014.2743291
GCGCTAG4355.1528314E-614.0310481
GTGTAGG33600.013.9872021
TCTATAC28900.013.9388033
GTGTTAG26700.013.7157451
GTCCTAA23150.013.709821
Produced by FastQC (version 0.11.5)