FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404605

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404605
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6371139
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCTA49300.030.003302145
GTACTAG13900.019.7826461
GCCGTAT14007.9853635E-1018.313444145
GTCCTAG25450.017.7676751
GTCCTAA25750.017.0860611
GTACTAA24100.016.9880851
CTAAGCG6201.8189894E-1216.7545854
CCTGTCG16503.929017E-1016.733963145
GTCTTAG25200.016.7315121
GTATTAA44050.016.6464651
GTATTAG24100.015.4667641
GTATATA47600.015.27661
GTTCTAG29350.015.1985241
CGACTAT7950.003953011714.884658145
GCGCTAA4605.6597855E-714.61241
CGACGAA8200.00485587214.430858145
GTGCTAA25500.014.1383441
GTATTAT43150.014.0197761
TATACTG34500.013.8150355
GTACGCG3100.001136943613.798221
Produced by FastQC (version 0.11.5)