FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404607

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404607
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8830871
Sequences flagged as poor quality0
Sequence length35-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG11000.019.4760991
GTTCTAG25500.017.6159521
GTATAGG14700.015.74926951
GTATTAA30950.015.4071171
GTACTAA19100.015.1966861
GTCCTAG22400.014.8090151
GTATTAG21050.014.1172241
GTCTTAG19850.014.100271
TATACTG29000.013.9067955
GTATAGA18400.013.8968651
GTGCTAG23200.013.8515361
CTTAACG5000.013.8016893
GTATATG25900.013.6082841
CTAAGCG5600.013.5480124
GTATATA35350.013.48941
GTACAGG33850.013.1684291
GTGTTAG21800.012.9975141
TCTAGAC21100.012.9186433
GGTCTAA18250.012.87505151
GCCTTAG21750.012.8685241
Produced by FastQC (version 0.11.5)