FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404608

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404608
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8724822
Sequences flagged as poor quality0
Sequence length35-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG11850.016.8707261
GTACTAA17100.016.528821
GTCCTAA18850.015.9086041
GTCTTAG20450.015.8437761
GTATTAA29950.015.0764341
GTATTAG18950.014.9151891
GTATATA36850.014.3112661
GTACTAT18800.013.9341311
GTACAGG37850.013.7510281
TATACTG28600.013.6191185
GTGCTAG24550.013.6189741
GTATACG3801.3224053E-913.6060411
CTAGCGT3355.0966264E-813.376124
GTCCTAG21050.013.2634681
TAGTGCG4253.8380676E-1013.20731457
GTATTAT28850.012.6643871
CTAGTAC10100.012.6271973
TCTATAC19450.012.5824543
TCTAGAC20300.012.5649953
GCTCTAG23150.012.5069791
Produced by FastQC (version 0.11.5)