FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404610

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404610
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8845717
Sequences flagged as poor quality0
Sequence length35-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG10650.019.09931
GCGTTAG3058.712959E-1015.8249831
GTATTAG19500.015.7351341
GGTCTAG18400.014.4273971
GTCTTAG20350.014.4002341
GTACTAA18850.014.2658721
GTGCTAG20850.014.2202581
GCCCTAG19200.014.1853781
TCTATAC21100.014.0516913
TATACGC2751.0882595E-613.7902645
GTTCTAG26350.013.7380071
GTCCTAG23100.013.7306441
GTATTAA29900.013.721161
TTATACT31650.013.3981254
GTATATA35000.013.2978311
GCGCTAG2609.240737E-613.2599441
GTATAAG18550.013.1956111
GTACATG34550.013.1716741
GTACATA26200.013.1587231
TATACTG27800.013.0213835
Produced by FastQC (version 0.11.5)