FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404611

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404611
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8845717
Sequences flagged as poor quality0
Sequence length35-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG12550.018.9727121
GTCCTAG21850.018.7940161
GCGATCG3200.018.31524537
GTCTTAG20800.017.0882641
CGATCGT3700.016.7741738
CGAATCG3501.8189894E-1216.7428139
GTTCTAG26200.015.41021251
GTATTAG18900.015.3370391
GTATTAA29500.014.973111
GTACTAT17900.014.844371
GTGCTAG21550.014.7320981
GTACTAA18900.013.6937851
TCTGTAC38550.013.5930173
GTCTTAT23600.013.159961
GTATATA35150.013.057211
GTGTTAG23800.013.0493721
TCTATAC19350.013.0058423
CTATACT22350.012.8012694
GTCTATA17100.012.7135991
GCGCTAA2457.7170625E-512.6765331
Produced by FastQC (version 0.11.5)