FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404613

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404613
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8606616
Sequences flagged as poor quality0
Sequence length35-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG97240.11298284947301007No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCTAG20750.017.6204221
GTACTAG12350.016.7576391
GTTCTAA24850.015.8236621
GCTCTAG23400.015.7723511
GTTCTAG24000.015.66548251
GTCCTAG21150.015.3301371
GTATTAG19400.015.2906261
CGAATCG3252.1518645E-914.8444559
GTCCTAT18550.014.1318241
GTATATA33500.013.7971231
GTATTAA29500.013.7971221
GTATATG26150.013.5860771
GCTCTAA23650.013.417921
CTATACT21250.013.1361594
GGTCTAA16450.013.0003271
TATACTG28500.012.9381195
GTACATA25750.012.8594541
CTAGGCC26050.012.8323544
GGTCTAG15400.012.7668171
GTATTGG22700.012.7638581
Produced by FastQC (version 0.11.5)