FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404614

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404614
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3105311
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGCG6150.027.11303145
CGTCTTA8000.025.374258145
GTACTAA9250.023.5188871
GTATTAG10800.022.8292881
GTCCTAG12400.022.8076291
TAATCGA3208.4665226E-720.3946275
GTTCTAG13400.019.4820611
GTATTAA16400.019.4556641
GTCCTAA11350.019.1673761
GTACACG6500.018.9658721
GTACTAG5901.2732926E-1118.4364151
GTATATA17250.018.0765931
GTCTATG15350.017.9519841
CGTCTTG13750.017.92675145
GTACAGG24300.017.9053251
GTATTGG13050.017.781841
TCTCGTT12450.017.46937145
CTATACT12050.017.4515574
GTTCTAA15000.016.9205321
GTGCATA12450.016.8914091
Produced by FastQC (version 0.11.5)