FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404615

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404615
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3105311
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGTT3100.056.118397145
GTCCTAG12600.028.2623861
CCGTATA5000.026.095053145
GACGATA8250.025.48002145
GTACTAA9950.024.8335671
CGCCGTT4201.8189894E-1224.162086145
GTACGGA3904.8930815E-1022.361451
GTATTAA17000.022.229911
TTATACC22750.020.391739145
GTCTAGG11300.019.9373071
GTATATA18250.019.5126611
GTGCTAG12700.019.456221
GTATAGG9000.019.3799211
GTACATA14050.019.1385361
GTCCTAT10450.018.7771971
CTAGCGC2000.00789238618.1358783
GCTCTAG14450.018.105811
GTCCTAA11500.017.6947121
GTTCTAA14000.017.6495721
GTATTAT14700.017.3035011
Produced by FastQC (version 0.11.5)