FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404616

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404616
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3051033
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCGT1450.001226806125.0007484
CTATACT11450.024.0618554
GGTCTAG9950.021.8585191
GTCTTAA14300.021.7999231
TAGCGCG8550.020.352154145
GCTCTAG14400.019.6347161
GTCTAGC8500.019.616951
GTTCTAG13750.019.5083961
GTCCTAG12000.019.3326451
CGAATCG2659.34377E-519.1546559
GTACGGT2701.080387E-418.7956281
GTATATG14550.018.435771
GTATTAG11100.018.2876381
GTATAGG8750.018.2279221
GTCTTAG12350.018.1977331
CTAGGCC15200.018.1255444
TAGTCCG2000.00791833518.1255425
GCTATAC7650.018.0070743
TCTAGGC15750.017.9529193
ACGCTAG2050.00913244517.6822971
Produced by FastQC (version 0.11.5)