FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404620

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404620
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2956734
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG10550.021.9912381
GTACTAA9300.021.0490741
GTCTAGG12100.020.971761
GTACTAG6250.020.8806821
GTGCTAG12000.019.3339651
GTTCTAA15600.019.055111
GTATTAA16800.018.9887161
GTATAGG7700.018.8317851
GTATAGA10800.018.796911
GTACTAC7750.018.710291
CTATACT11300.018.6077484
GTATTAG9750.018.5903511
ACGTAAC2400.001059784418.1265133
CGTCTTG18500.018.02731145
TCTAACC9750.017.8476433
GTATATA16000.017.6724511
GTCTTAA12550.017.3312441
GTTCTAG13550.017.122331
CCGTTAA2550.001591641517.0593811
GTACAAG18950.016.8343241
Produced by FastQC (version 0.11.5)