FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404622

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404622
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10980945
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG20850.017.3446161
GTACTAG12850.016.6176661
GTCCTAG29650.016.6108861
GTACTAA21150.016.1215321
GTATTAA31850.016.1123281
GTGCTAG28950.015.2281
GTTCTAG31700.014.6674961
GTCTTAG24050.014.0343541
GTCCTAA22250.013.9313751
TCTAGAC24000.013.6335713
GTCTAGG27450.013.2997961
GTATAAA40500.012.9261751
CTAGACA32500.012.8236354
GTATATG31600.012.7520561
GTTCTAA32800.012.7055361
ATAGTAC20350.012.6938723
GGTCTAA20150.012.6484741
TCAGTAC31400.012.5046783
GTACAGG48400.012.38184451
TAGACAG41850.012.3456185
Produced by FastQC (version 0.11.5)