FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404623

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404623
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10980945
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG22850.016.781621
GTACTAG13700.016.1382521
GTCCTAG30200.015.9002871
TACGCGA2201.6162303E-615.6886592
GTATAAG18950.014.7663421
GTACTAA21500.014.6217731
GTTCTAG31800.014.2311931
GTCCTAT22900.014.1804321
GTCTTAG25150.013.8733231
GTGCTAG28300.013.7939651
GTCCTAA24150.013.7325511
GTTCTAA31450.013.6206591
GTATAGA23950.013.4145021
GGTCTAG22850.013.0019761
GTACAGG48200.012.9009771
CTATACT23800.012.7445344
GTATATA40250.012.7026091
GTGCTAA27600.012.6418141
GGTCTAA19000.012.5456341
CTAGACT24050.012.4687374
Produced by FastQC (version 0.11.5)