FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404624

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404624
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11139758
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG14350.016.8034321
GTTCTAG32200.015.8328011
GTCCTAG28150.015.4185671
GGTCTAA20550.015.0862941
GTATAGG18000.014.7356781
GTACTAA21300.014.5550861
GCCCTAG27550.014.3789821
GTATATA40200.014.138711
GTCTTAG24800.014.0288331
GTATAAG21300.013.746471
GTATTAA31350.013.5151051
GTGCTAG28600.013.1284161
GTATTAG23050.013.0017021
GCCTTAG26450.012.8932211
CGAATCG5451.8189894E-1212.6416839
CTAGACT23300.012.4192324
TCTATAC24700.012.412483
GTACAGG48600.012.4037691
GCGCTAG3902.7956048E-812.3656031
TCTAGAC24500.012.2325973
Produced by FastQC (version 0.11.5)