FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404625

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404625
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11139758
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG28300.018.0710811
GTACTAA19000.016.0043471
GTACTAG13050.015.3577061
GTATTAG22400.015.2720051
GTGCTAG29950.014.9987611
GTCTTAG27450.014.72829151
GTTCTAG31050.014.2448291
GCGCTAG4202.7284841E-1113.9864841
CTATACT25750.013.6544174
GTCTTAT30100.013.5464151
GTCCTAA25300.013.3848791
GCTCTAG31550.013.3619361
GTATTAA32900.013.3387981
TCTATAC24750.013.2422463
GCCTTAG26350.012.9826541
GTTCTAA33750.012.9004741
GTATAGA25450.012.8986631
GGTCTAG23900.012.7231221
GTATACG4357.039489E-1012.70982551
GTCTTAA29200.012.6622191
Produced by FastQC (version 0.11.5)