FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404626

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404626
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10976996
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG12350.016.4566861
GTTCTAG30450.014.3672531
GTCCTAG28300.013.8763661
GTATTAA31300.013.8670321
GTATTAG22000.013.7789881
GTACTAA20550.013.7454611
GCTCTAG33300.013.6548531
GTGCTAG28700.013.2028621
GGTCTAG20900.013.1856341
GTACTAC16250.012.931051
TATACTG30400.012.5796935
GTCCTAA25700.012.4654271
GCCCTAG28250.012.3157331
GTGCAGG100800.012.3026671
GTACATG39450.012.1373841
GTATAGG16050.012.0190551
GTCTAGA24100.012.0065861
TCTGTAC45750.011.9732713
GTCTTAA30850.011.9477451
GTACAGA51450.011.7837791
Produced by FastQC (version 0.11.5)