FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404629

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404629
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10900700
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG12550.015.9279251
GTCCTAG30050.015.7127081
GTGCTAG27650.014.8329231
GTATTAG22700.014.72722151
GTATAGG18200.014.20251
GTCCTAA24000.014.0730991
TATACTG29850.013.9626835
GTTCTAG29900.013.8320011
GCGCTAG4751.8189894E-1213.7858941
GTCTTAG24800.013.75811
CCTATAC17750.013.5853663
GCTCTAG33000.013.5770161
GTACTAA21350.013.3984681
GTGCTAA26950.013.2999341
GTTCTAA31450.013.1502971
GGTCTAA19950.013.1294231
TCTGTAC47550.013.1129293
CTGTACT52350.013.0937564
CTAGACC21850.012.7691344
CTATACT24500.012.6533134
Produced by FastQC (version 0.11.5)