FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404630

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404630
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5602291
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCTA39000.032.9025145
GGGGGGG362300.018.911924145
GTATTAG20200.017.6493911
GTATTAA27350.015.9788551
TCTAGAC18050.014.9730433
CTAGTAC10400.014.9286253
GTACTAA15050.014.9010721
GCTCTAG25000.014.490721
GTACAGA40500.014.4822021
GTCCTAG20750.014.4103541
GTATAGT15700.013.9178891
GTCTTAG20050.013.766271
CCTGTCG17706.693568E-613.678686145
GTGCTAA21600.013.5770641
GTACTAG10250.013.4640841
GTGTATG30850.013.4204411
GTTCTAG25500.013.3045821
TAGACAG32700.013.1891995
GTTCTAA26950.012.5887521
ATTAGAC17500.012.4863353
Produced by FastQC (version 0.11.5)