FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404633

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404633
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5553469
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCTA34600.028.028944145
GTCCTAG20100.018.926561
CCTGTCG15905.9299055E-918.298141145
GTATTAG18100.017.1964591
GTGCTAG20150.016.8772141
CGCCGTA13806.166374E-1015.81198145
GTATATG26350.015.5310761
GTCTTAG18600.015.4946171
GTACTAA14400.015.210551
GTCTAGG20900.015.1684141
GCTCTAG26050.015.0461371
GTTCTAG23450.014.747971
TATACTG24450.014.5966625
GTATAGA20200.014.5526671
CGGTGTA30050.014.522818145
GTACTAT15750.014.2727571
GTATACG4951.6396007E-713.9733281
GGTCTAG15850.013.8190491
GTTCTAA25650.013.7077521
TTATACT29100.013.6505954
Produced by FastQC (version 0.11.5)