FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404634

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404634
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5450049
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCTA41150.033.900394145
GGGGGGG292750.021.40285145
GTTCTAA26400.018.3339561
GTACTAG10350.016.1450161
GTTCTAG21250.015.9983021
TATACTG24950.015.9359995
GAGACGT56200.015.566395145
TCGTATG20000.015.368658145
CTATACT19500.015.3660234
CCTATAC15750.015.3658823
GTATTAA26750.014.6475821
GTCCTAG20800.014.40525151
GACGTAT10050.005620240714.115875145
GCGCTAG4508.5712963E-714.08513451
GTCCTAT17350.013.94861
GTCCTAA17150.013.7752841
GTCTTAG21650.013.0412441
GCCTTAG20850.012.9889081
TAGTACT16100.012.8845554
GTGCTAA20450.012.6794381
Produced by FastQC (version 0.11.5)