FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404636

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404636
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5291179
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCTA41000.044.334026145
GGGGGGG259750.024.582237145
CGCGTAT5403.4567576E-521.577576145
GTATTAG18250.017.9900991
GTATTAA26350.016.6132491
CCGTCGG9902.9549462E-416.477423145
GTCTTAG18650.016.3688661
GCTCTAG23350.015.0475041
GTACTAA16100.015.0260551
GTTCTAG23450.014.4920811
GTACTAG10650.014.0619411
GTTCTAA22750.013.6720591
GTATACG4651.245462E-613.6257781
GTCCTAG19450.013.3264491
GTATAGG18100.013.0474861
CAGTACT32050.012.9397544
GTGTTAG21450.012.8894831
TGTACCG9400.012.8680585
GTACTAT15700.012.8407371
TATACTG22550.012.7715915
Produced by FastQC (version 0.11.5)