FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404637

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404637
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5291179
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATA6651.02063495E-832.190323145
CTGTCTA38550.032.083626145
GCCGTAA7701.3164954E-624.711763145
GCCGTAT18650.021.680763145
CCTGTCG22200.019.285192145
GTATTAG17450.018.8065681
GTACTAG10700.017.756621
GTCCTAG20850.016.56821
TACACAA45000.016.385271145
TCGCCGT24250.015.693242145
GTGCTAG18950.014.5835131
GGTCTAG17850.014.5145781
GTTCTAG22250.014.4906591
GTCTTAG19800.014.2482341
GTATAGG14450.013.9453781
GTTCTAA23450.013.7491321
CGGTGTA40500.013.507571145
GTATTAA27000.013.4340471
TTAGACG42500.013.43157145
CTAGTAC10300.013.41008953
Produced by FastQC (version 0.11.5)