FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404638

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404638
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5453777
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG11950.023.667571
GTATAGG14250.018.8297181
GTCCTAG19450.018.6426371
CTAGACT17400.018.3358334
TCCTATG62700.017.80716145
GCCCTAG20900.017.6962341
CTATACT20450.017.3740064
GTACAGG36200.017.0281451
GTGTTAG22250.016.9484621
TATACTG26400.016.7543325
GCTCTAG23850.016.7236581
AGACGGC58500.016.359104145
GTATATA31950.016.1155241
GGTCTAA16800.015.9716361
GGGGGGG3206150.015.94893145
GTATTAG21950.015.8585561
CGTCTTG30700.015.8226145
TGCACCG19300.015.7794985
CACGAGC35000.015.742983145
GTGCTAG21250.015.6984171
Produced by FastQC (version 0.11.5)