FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404639

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404639
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5453777
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATA9800.022.182062145
GTCTTAG18800.020.8590891
GTACTAG11750.019.7775081
GTCCTAA18400.019.3391651
GTTCTAG23800.019.2230821
GTCCTAG19550.018.2015671
GCCCTAG19350.017.2637981
GTATTAA32100.016.9674151
GTCCAGA51500.016.921291
TCTATAC20200.016.8714313
CGCCGTT15050.016.851488145
GTATACG6505.638867E-1116.7585851
CTATACT18650.016.7124354
GTACTAC17400.016.6943761
GTACTAA18450.016.5315041
GTGCTAG19550.016.3442631
GTCTATG24450.016.3359071
GCTCTAG24450.016.038891
GTACATG34900.016.0222951
CTGTACT39600.015.7417624
Produced by FastQC (version 0.11.5)