FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404640

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404640
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5451914
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG21350.021.0563771
CGTCTTG28750.019.67119145
GTCCTAG21500.018.8859751
GTACTAA17150.018.6028041
GTACTAG12200.017.8299981
GTCTTAG19600.017.387281
TCTAGGC23050.017.2985573
GTATAGA18950.017.2184141
CTAGACA21700.017.0383914
GTGTATA23000.017.0237711
TCTAGAC19750.016.8853033
TCTATAC20500.016.6211913
GTTCTAG21100.016.494861
GGTCTAA16450.016.3089371
GTATATA29100.016.1960931
GTGCTAT23450.016.0786781
GTATTAA31400.015.933431
GTGTAGA30950.015.93081951
GGGGGGG3246400.015.799267145
CTAGACT17900.015.7953684
Produced by FastQC (version 0.11.5)